[[en/index|English]] · [[zh/index|中文]] · [[en/classics/oneill-silica-fibers|EN]] · [[zh/classics/oneill-silica-fibers|中文]] # O'Neill 系列论文要点 O'Neill 等人关于二氧化硅纤维促癌的系列论文，其主要内容与图表数据归纳如下。 ## 1973 生物质硅纤维促细胞增殖的“锚定机制” - Maroudas, N. G., C. H. O'Neill, and M. F. Stanton. "Fibroblast anchorage in carcinogenesis by fibres." The Lancet 301.7807 (1973): 807-809. 提出纤维致癌的"锚定依赖性生长"假说。 机制：长纤维通过提供物理锚定点，激活间质细胞（如成纤维细胞）的持续增殖，导致间皮瘤。 体外实验： ![[About O’Neill fig2.png|400]] The ability of fibers to support cell growth increases starting from a length of 40 μm. 从40μm开始纤维支持细胞生长能力增强 体内实验： ![[About O‘Neill fig1.png|400]] A higher incidence of mesothelioma was associated with a higher proportion of long fibers in the sample. 发病率越高，样本中长纤维的比例越高。 ## 1980 伊朗面粉中的生物质硅刺污染物 - O'neill, C. H., Hodges, G. M., Riddle, P. N., Jordan, P. W., Newman, R. H., Flood, R. J., & Toulson, E. C. (1980). A fine fibrous silica contaminant of flour in the high oesophageal cancer area of North‐East Iran. International Journal of Cancer, 26(5), 617-628. A silicon fiber contaminant was discovered in flour from a high-incidence region of esophageal cancer in Iran. This fiber originates from the inflorescence bracts of Phalaris minor. 在伊朗高食管癌高发区的面粉中发现了一种硅纤维污染物，该纤维源自小籽虉草（小麦种植区的杂草）的种子苞片。 ![[About O’Neill fig3.png|500]] In vitro test confirmed that cells attached to silica fibers from Phalaris canariensis proliferate significantly faster than cells suspended in gel. 体外实验验证附着在 Phalaris canariensis 硅纤维上的细胞增殖速度远快于悬浮在凝胶中的细胞。 ![[About O’Neill fig9.png|400]] ## 1982 中国林县食物及癌旁组织的生物质硅刺污染物 - O'Neill, C., Clarke, G., Hodges, G., Jordan, P., Newman, R., Pan, Q.Q., Liu, F.S., Ge, M., Chang, Y.M. and Toulson, E., 1982. Silica fragments from millet bran in mucosa surrounding oesophageal tumours in patients in northern China. The Lancet, 319(8283), pp.1202-1206. Mucosa adjacent to tumors from Linxian surgical cases was compared with normal mucosa from London controls. 林县食管癌患者手术切除的癌旁黏膜与伦敦尸检正常食管黏膜作为对照。 <table> <tr> <td> <img src="About O’Neill fig4.png" style="height: 200px; width: auto; display: block; margin: 0 auto;"> </td> <td> <img src="About O’Neill fig10.png" style="height: 200px; width: auto; display: block; margin: 0 auto;"> </td> </tr> <tr> <td align="center"><em>a：收集林县样本残渣所用的 25 mm 滤膜表面。</em></td> <td align="center"><em>b：同倍率下伦敦肺炎患者的相似样本。</em></td> </tr> </table> The concentration of silica particles found in the esophageal mucosa of chickens from Linxian was similar to that in patients from Linxian. 林县鸡食管黏膜中发现的二氧化硅颗粒浓度与林县患者相似 ![[About O’Neill fig5.png]] ## 1984 生物硅纤维促进小鼠皮肤癌变 - Bhatt, T., Coombs, M., & O'Neill, C. (1984). Biogenic silica fibre promotes carcinogenesis in mouse skin. International journal of cancer, 34(4), 519-528. Dietary exposure: Adding Phalaris canariensis seeds containing silica fibers to the feed resulted in a significant increase in skin tumor incidence around the mouth and nose of mice. 饮食暴露：将含硅纤维的金丝雀虉草籽粒加入饲料，小鼠口鼻周围皮肤肿瘤发生率显著升高。 ![[About O’Neill fig6.png]] Skin contact studies: 1) Dorsal skin exposure to seeds via a wire gauze barrier; 2) Light rubbing of dorsal skin with isolated fibers. Both methods resulted in frequent tumor development at the contact sites on the dorsal skin. 皮肤接触：1.通过金属网屏障让小鼠背部皮肤与籽粒接触，2.用纯纤维轻轻摩擦起小鼠背部皮肤，小鼠背部摩擦部肿瘤多发。 <table> <tr> <td> <img src="About O’Neill fig7.png" width="100%"> </td> <td> <img src="About O’Neill fig8.png" width="100%"> </td> </tr> </table>